FAQ's about deconvolution

Deconvolution

• What is the potential danger to apply the non-negativity criterion between iterations? Answer.
• Can I deconvolve a single 2D Tiff image? Answer.
• Can artefacts due to unstable laser power be removed by deconvolution? Answer.
• How should I interpret the Quality factor? Answer.
• Up to which depth would Huygens restore 2-photon data? Answer.
• What is the computation time for deconvolving an image? Answer.
• I am not getting much compression of the z-blur with a measured PSF, why? Answer.
• Is it possible to deconvolve brightfield images? Answer.
• Skipping channels when deconvolving with Huygens Pro? Answer.
• Where can I find more references and support about deconvolution? Answer.
• Can I compare the quality factor and the I-divergence of the deconvolutions of different images?Answer.
• Can confocal reflection images be deconvolved? If so, how? Answer.
• Can I deconvolve a single widefield image plane? Answer.
• Why do my deconvolved images look darker than the raw images? Answer.

• Can I deconvolve images acquired with a TIRF system? Answer.
• MLE versus ICTM - Will one method be faster or more rigorous or give a better quality result? Answer.
• Time Series: Does Huygens load the whole time-lapse at once? Answer.
• MLE versus ICTM - Which method is more effective under certain circumstances? Answer.
• How large a resolution improvement can be expected from the Huygens deconvolution?? Answer.
• Which deconvolution method to use? Answer.
• Can a 2D-time series be deconvolved as 3D stack? Answer.
• Is deconvolution on 2D or 2D-time images possible? Answer.
• Can a small sphere-like object look again like a sphere in X Y Z after deconvolution? Answer.

Miscellaneous

• Is the dimension after deconvolution fixed according to voxel dimension/aspect ratio? Answer.
• Memory allocation error while running ICTM; what to do? Answer.
• Are there batch processing scripts available? Answer.
• How do I tell Huygens that I am using a 2-photon system? Answer.
• How to limit memory needs? Answer.
• Intensity spreads over the image corners after Fourier based convolution. Why? Answer.