Restoration Parameters

Also called Deconvolution Parameters in the Huygens Software , the Restoration Parameters are those necessary for Doing Deconvolution , together with the Microscopic Parameters that describe the image.

Most general

• Restoration Method
• Point Spread Function image (if not provided, a theoretical PSF will be used instead based on the Microscopic Parameters ).

Background

• Background Mode
• Background Per Channel

Iterations control

• Maximum number of iterations
• Signal To Noise Ratio
• Quality Change Threshold
• Iteration Mode
• Bleaching correction
• Brick layout

For scripts

• Time Out

A typical example for a two-channel image could be:

Restoration Method Classic Maximum Likelihood Estimation
Point Spread Function theoretical
Signal To Noise Ratio { 14 7 }
Background Mode Manual
Background Per Channel { 900 2400 }
Bleaching Mode Off
Brick layout Auto
Quality Change Threshold 0.003
Max Num Of Iterations 80
Iteration Mode Fast
Padding Mode auto
Time Out 6000

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