Multi channel images

Fluorescence Microscopes can usually register different Emission Wavelengths (almost) simultaneously, allowing you to image different dyes on the sample. In the terminology of the Huygens Software , one channel in a 3D image refers to the intensity distribution recorded at a given fixed wavelength, independently of what device made the acquisition. Thus, it is a logical channel of stored data, and not necessarily a physical channel (as all the image channels could have been measured by a single photomultiplier, for instance).

In the Huygens Software , every channel is therefore deconvolved independently, using specific Microscopic Parameters (in an ideal Cross Talk free situation there's no correlation between channels). A multi-channel image requires a multi-channel Point Spread Function .

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