SVI Movie Gallery 1
Jurkat T-cell
making contact with a Raji B-cell, stained for Raji cytosol (FURA-2, blue), T-cell receptor (anti-CD3 Alexa647, green), and Actin (Lifeact-mRFPruby, red).
Animated SFP rendering of a deconvolved time series. Original data was imaged using a Zeiss Cell Observer HS system with a 40x Fluor 1.3NA oil lens and deconvolved using Huygens Essential. The original time interval is 1 minute. "We look at the molecular events and redistribution of certain markers for T-cell activation like the TCR (green) and Actin (red). In the time series a ring-like pattern of Actin and a bulls-eye pattern of CD3 can be seen, accumulating at the contact-site (the immunolgical synapse. Both structures gave us a hint that the T-cell was activated properly." Recorded by Christian Junker M.Sc., Institute of Biophysics, University of Saarland, Germany.SFP Rendering of a cell cluster.
Imaged using an inverted Leica TCS SP2 AOBS confocal microscope and deconvolved using Huygens Professional. The original data was recorded by Dr. Nicolas Fete, Laboratory of Stem Cells Dynamics, Swiss Federal Institute of Technology of Lausanne, Switzerland.Multiple renderer animation made with the new Huygens Movie Maker.
The data shows an isolated rat hepatocyte couplet. Recorded by Dr. Permsin Marbet in the lab of Prof. Lukas Landmann, Department of Anatomy, University of Basel, Switzerland.