Experimental PSF with the Huygens PSF distiller
While your data will always automatically get a theoretical (als called automatic, on the fly, calculated) PSF based on the parameters, without any work to be done from your part, Huygens also offers the PSF distiller. This Option is present in the Huygens Software that allows you to obtain an experimental or distilled Point Spread Function (PSF) from bead images obtained (Recording Beads). You can use multiple beads recorded as separate images, multiple beads in the same image, or both: the wizard guided tool will assist you in properly distilling the PSF from single and multiple channel data.
Chromatic shift, if present, will be reported and can be corrected with the Chromatic Shift Correction tool.
The PSF distiller uses a Non Linear Iterative Method to measure a PSF when your beads are not small enough to provide a direct measurement of the PSF (i.e. they are not Sub Resolution in terms of Band Width).
To learn how it works, refer to Psf From Beads. For a practical tutorial see Using The Psf Distiller.
You can Test The Software to see how it works.
