Print

Huygens Options


The Huygens basics include the following visualization tools: (Twin)-slicer, Ortho-slicer, SFP volume renderer, MIP-renderer. To enable the deconvolution you must add minimally ONE optical Option.

Optical options

Each optical option is specifically designed to take into account the optical properties of that type of microscope for deconvolution:
Huygens Confocal option More ».
Huygens Widefield and Brightfield option More ».
Huygens Spinning Disk option More ».
Huygens Multi Photon option More ».
Huygens STED option More ».

Functional options

Huygens Time Series option- Allows automated deconvolution of time series. and correction of time-dependent background, bleaching and z-drift.More ».
Huygens Extended File Formats option Support for extended input file formats. More »

Server options

Huygens uses by default up to 4 CPU cores and one socket (desktop version). To speed up the Huygens software, one of the following server option can be added to your license. CPUs can be either single or hyper-threaded (32-64bit).
Small Server Up to 16 CPU cores and two sockets.
Medium server Up to 48 CPU cores and four sockets.
Large server option Up to 128 CPU cores and eight sockets.
Extra Large server More than 128 CPU cores and more than eight sockets.

Restoration Tools

Screen shot Description
Image The PSF Distiller.

The PSF Distiller is a wizard guided option that can be used to obtain an experimental or distilled Point Spread Function (PSF) from acquired single and multi-channel bead images.Chromatic Shifts will be automatically reported and can be corrected by theChromatic Shift Corrector.
Image The Chromatic Shift Corrector.

The Chromatic Shift Corrector estimates and corrects for chromatic shifts between different channels of multi-channel images, removing the existing misalignments across different channels. The result of this correction is a channel-aligned image free of chromatic shifts.


Image The Object Stabilizer.

The Object Stabilizer can measure and correct for cell motion, thermal drift, shaking, and other types of movement (x-y-z translation and axial rotation). Both the measurement and subsequent stabilization are done in 3D and at sub-pixel level. The Stabilizer stabilizes 2D or 3D time series, and slices within a 3D stack.


Visualization Tools

Screen shot Description
Image The Twin Slicer.

The Twin Slicer allows you to synchronize views of two images, measure distances, plot line profiles, etc. In Basic Mode, image comparison is intuitive and easy, while the Advanced Mode gives the user the freedom to rotate the cutting plane to any arbitrary orientation, link (synchronize) or unlink viewing parameters, and more.
Image The Orthogonal Slicer.

The Orthogonal Slicer is designed to show the same point in 3D space from 3 orthogonal directions, i.e., xy (top left), xz (bottom left), and yz (bottom right). If you move one of the slices, the others will follow to make sure that the center of each of the slices intersects in the same point in space. This behavior makes the Ortho Slicer a useful tool to study small objects in 3D.
Image The SFP Renderer.

The Simulated Fluorescence Process renderer considers a 3D microscopy image as a distribution of fluorescent material. The SFP algorithm creates depth cue rich images from unprocessed data, and is eminently suited to render 3D microscopic data sets since it does not rely on boundaries or sharp gradients. Because the algorithm is based on ray-tracing, it does not require a special graphic card.
Image The MIP Renderer.

The Maximum Intensity Projectionrenderer projects only the voxels with maximum intensity that fall in the way of parallel rays traced from the viewpoint to the plane of projection. This allows you to obtain a direct spatial projection of your 3D microscopy data from the point of view you wish.
Image The Surface Renderer.

The Surface Renderer renders very large microscopic volume data to high resolution output images using fast Ray Tracing algorithms. Separated volumes are represented continuous surfaces and thus can be clearly distinguished from one another.
Image The Movie Maker.

The Movie Maker is a tool that allows you to easily create sophisticated animations of your multi-channel 3D images using the powerful Huygens Visualization renderers.Movies can be exported as AVI or TIFF images.


Analysis Tools



Screen shot Description
Image The Colocalization Analyzer.

The Colocalization Analyzer provides quantitative information about the amount of spatial overlap between different data channels, in 3D stacks or 3D time series. Huygens gives you the colocalization coefficients most commonly used in literature: Pearson, Overlap, Spearman, and Manders M and K. An optional 'Replicate-Based Noise Corrected Correlation (RBNCC) tool can be added to correct the contribution of noise to the Pearson and Spearman coefficient.
Image The Advanced Object Analyzer.

The Advanced Object Analyzer is a great tool to obtain statistics of individual or all objects with a single button press. With the 3D region of interest (ROI) selector tool you can limit the analysis to a certain volume, but also crop your original data in 3D for further analysis. Objects can be analyzed in different channels and at different time points, and their statistics can be exported as CSV files.
Image The Object Tracker.

The Object Tracker is equipped with a wizard for optimized object detection and fast track filtering. The integrated Track Analyzer analyzes object position, speed and flow, and enables the user to visualize and export results in histograms and plots, and as CSV files.