Zeiss LSM700

This is part of the Backprojected Pinhole Calculator .

Details

The microscope reports the side length (s) of a square pinhole in microns (µm). Use this reported value in the form below.

Because of the square pinhole, the shape factor is c = 1/sqrt(π) ~ 0.5642. The system magnification is reported to be 1.53. The simplified equation to calculate the Back Projected Pinhole Radius in nanometers is therefore r_b ~ 564 s / (1.53 m_obj).

Calculator

Pinhole side (microns)
Objective magnification

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