3D Microscopy

Optical concepts

• 3D Microscope
• Fluorescence Microscopes
• Image Formation
  • Numerical Aperture
  • Point Spread Function (PSF)
• Sampling Density
  • UnderSampling and Nyquist Rate
  • Anti Aliasing and Aliasing Artifacts
• Refractive Index Mismatch
  • Spherical Aberration: Mismatch Distorts Psf
  • Fishtank Effect
  • Total Internal Reflection

Other concepts

• Clipped Images
• Bleaching Effects
• Pinhole Radius
• Optical Effects
• Other Optical Concepts

Microscopical image processing

• 3D Image
• BackGround vs Black Level
• Signal To Noise Ratio

• Recommended Reading