Enable depth-dependent PSF correction
The Huygens Software can adapt the theoretical Point Spread Function (PSF) to the sample depth to (partially) correct for spherical aberration while doing deconvolution if a mismatch in the refractive indices is present. In case of such a mismatch, the HRM will let the user to decide whether a correction should be performed or not.
If the user answers 'No', the HRM will create a static PSF at the coverslip (z = 0) and use it for deconvolution: no depth-dependent correction will be performed. The PSF will be assumed to stay perfectly preserved at any distance from the coverslip.
If the user instead chooses 'Yes', additional options for correction will be presented.