Zeiss LSM510

This is part of the Backprojected Pinhole Calculator .

Details

The size of the circular pinhole is given by the microscope as a diameter d expressed in microns (µm). Use this value in the form below.

The system magnification is reported to be 3.33. The simplified equation to calculate the Back Projected Pinhole Radius in nanometers is therefore r_b ~ 500 d / (3.33 m_obj).

Calculator

Pinhole diameter (microns)
Objective magnification

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