How to limit memory needs?
These steps will help to lower the amount the memory needed for deconvolution:
- Switch off the undo system (Options menu).
- Reduce the size of the image (Crop tool). In particular, widefield images often contain many slices with blur.
- Switch off padding during the deconvolution of confocal images. This might produce edge artifacts, but it shouldn't be a problem on most confocal images.
- A theoretical PSF will be generated on-the-fly by the MLE and QMLE restoration methods. There's no need to create a theoretical PSF by hand.
Keywords: undo padding
Categories: Faq Deconvolution, Huygens Faq, Imported Faqs
Platforms: Irix Linux
Related products: Hu Pro
