How much undersampling is still ok?
Theoretically the voxel size for the used microscope (Leica TCS) should be somewhere 40x40x50. Is this really necessary since this would require very large image sets.
In typical conditions (1.3 lens, 488 excitation) good sampling is around 50nm x 150nm. Laterally 70nm is fine, you shouldn't go beyond 100nm, axially it is best to stay at 150-200nm.
If you sample around the 70nm x 150nm you will see that the restoration can reduce noise considerably. As a result you might need less signal than you thought before. If practical considerations (bleaching, data size) don't allow these sampling densities, you'll just have to do the best you can. In our experience, unless you undersample dramatically, the restoration will always improve your image.
See UnderSampling.
Keywords: undersampling sampling typical conditions
Categories: Faq Deconvolution, Faq Microscopy, Huygens Faq, Imported Faqs
Platforms: Irix Linux Windows Mac
Related products: Hu Ess Hu Pro
