What is an undersampled stack of images?
Is a stack called undersampled when the Z intervals between images, i.e. the voxel height, is too large with regard to the objective magnification and numeric aperture?
An undersampled image stack is a stack in which the Z or XY samping intervals are too large. <br>
Undersampling means that the sampling interval is too large to capture all information about the object generated by the microscope. In Huygens Essential the sampling values will be colored orange in case of undersampling. In case of severe undersampling the color will be red. <br>
In Huygens Pro the optimal sampling density for the optical conditions under which an image is recorded can be computed by selecting its thumbnail and then Edit->Nyquist. When an image is recorded at this so called Nyquist frequency the digitized sequence contains *all* information carried in the signal. This makes it possible to reconstruct the image at any location, so not limited to the sampling positions. The Nyquist frequency is twice the highest spatial frequency (bandlimit) transmitted by the microscope.
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Keywords: undersampled stack Nyquist sampling<br>
Categories: Faq Deconvolution, Faq Microscopy, Huygens Faq, Imported Faqs<br>
Platforms: Irix Linux Windows Mac<br>
Related products: Hu Ess Hu Pro<br>
