Are multiple images from the same specimen needed at different focusing depths?
I am interested in obtaining confocal-like images using a regular epifluorescence microscope. It appears that deconvolution is the solution.
If you want to obtain confocal-like 3D images from regular epifluorescence microscopy, i.e. widefield microscopy, then you need indeed a series of images recorded at different depth: a so-called stack. To arrive at a result that is comparable to a well-sampled confocal image a typical Z step size (Z sampling distance) is 200nm.
If you'd like to obtain confocal-like single slice images the best procedure is to <br>
acquire a short stack of 10-20 slices around the plane of interest and deconvolve that. <br>
However, if you lack a z-drive or the time to acquire the stack Huygens-Pro <br>
and Huygens Essential also allow you to deconvolve a single 2D widefield image.
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Keywords: single slice epifluorescence stack<br>
Categories: Faq Deconvolution, Huygens Faq, Imported Faqs<br>
Platforms: Irix Linux Windows Mac AIX<br>
Related products: Hu Ess Hu Pro<br>
