How does Huygens derive a Point Spread Function?
To obtain a measured PSF the Huygens Software contains a set of tools with which you can derive a PSF from images of fluorescent beads. In principle one would need a very small, point-like bead to measure a 'point' spread function. Since sufficiently small beads are also very dim this is impractical. Huygens solves this by being able to extract PSFs from larger, much brighter beads. A typical size is 200nm radius. To improve accuracy it is also capable of averaging over multiple beads, from single or multiple images.
See also Psf Distiller.
Keywords: PSF measurement beads
Categories: Faq Deconvolution, Faq Microscopy, Huygens Faq, Imported Faqs
Platforms: Irix Linux Windows Mac
Related products: Hu Ess Hu Pro
