The pinhole size of a measured PSF seems to have no effect on the the restored images. Why?
Varying the pinhole size in the parameters (and clicking the `accept' button) has no influence whatsoever on the quality factor of the reconstructed PSF, or on the quality factor of the restored images. Something is wrong!!!
No. The reason here is that the shape of the expected (theoretical) PSF is forced only lightly on the measured PSF. This is done in the form of a bandlimit constraint, and the pinhole size has not much influence on this. If we would impose the theoretical shape on the measured data more strongly it would, for instance, force a non-existent axial symmetry on the measured PSF, exactly what we don't want. Still, from version 2.07 of the Huygens onward the theoretical shape is imposed on the data slightly more strongly.
Keywords: pinhole size reconstructed PSF distiller<br>
Categories: Faq Deconvolution, Faq Microscopy, Huygens Faq, Imported Faqs<br>
Platforms: Irix Linux<br>
Related products: Hu Pro<br>
