Which pinhole size to use in case of mechanical hysteresis?
On my microscope the nominal pinhole sizes are not reliable. There is apparently a LOT of mechanical backlash in our system and the size looks as if it's varying by factors greater than 1.5, depending on if you're moving UP to a given iris diameter, or moving DOWN.
Start with the smallest pinhole. This gives the smallest PSF resulting in the most conservative restoration (less artifacts). If you are not satisfied with the resolution you may wish to increase the pinhole size.
Keywords: pinhole PSF artifact resolution
Categories: Faq Deconvolution, Faq Microscopy, Huygens Faq, Imported Faqs
Platforms: Irix Linux Windows Mac
Related products: Hu Ess Hu Pro
