SVI Image Gallery 3

Image	Description
	Endothelial cells labeled with WGA and an extra-cellular epitope antibody against P-selection Endothelial cells were labeled with WGA-alexa 488 and an extra-cellular epitope antibody against P-selection (secondary Ab Alexa 555). The z-stack was acquired using a 63x/1.4 oil Plan-Apo objective mounted on a confocal microscope. Subsequently, the 3D volume was deconvolved, iso-sampled and surface-rendered with Huygens Professional. Courtesy of Louis Villeneuve, (laboratory of Dr Martin Sirois), Montreal Heart Institute, Canada
	DiI-labeled spiny neurons. Neurons were labeled with the cyanine membrane dye DiI. Image stacks were recorded with a 1.4 NA oil immersion objective, and deconvolved using Huygens. The third panel shows the overlay of the DiI labeling and immunostainings against our molecules of interest: deconvolution grants us the resolution to investigate distribution of the signal within a dendritic spine. Image created by Barna Dudok MSc., Hungarian Academy of Sciences, Hungary
	Dissociation between dynein and dengue E protein at 72h. The image shows a Baby Hamster Kidney (BHK)-21 cell infected with Dengue virus-2 protein at 72 hours post infection. The cell was stained for dynein motor protein (involved in retrograde trafficking of proteins) with Alexa 488 (green) and Dengue virus envelope (E) protein with TRITC (red). The image was acquired with a Zeiss LSM 510 Meta. The Z stack image was deconvolved, and 3D reconstruction of the image was done using the Surface Render option of Huygens Essential. Image created by Dr. Cecilia Dayaraj, Dengue group, National Institute of Virology, Pune, India
	Metaphase cell before and after Huygens deconvolution. Cells were stained for a-tubulin (shown in green), cenp-A (red) and DAPI (blue). It can be observed that kinetochores (in red) attach to the microtubules. Stacks where acquired with a Delta Vision microscope and deconvolved using the Huygens Professional. A maximum intensity projection of the raw (left) and deconvolved (right) image is shown. Image created by Dr. Claudia Florindo, Microscopy Unit, Department of Biomedical and Medical Sciences, University of Algarve, Portugal
	Mob1 protein in Tetrahymena Tetrahymena was stained for centrin (red) and Mob1-GFP (green). Stacks where acquired with a Delta Vision microscope and images were subsequently deconvolved with Huygens. A surface rendered and MIP projection is shown. Centrin staining is present in the cilia of Tetrahymena, and Mob1 staining is found in the apical region of this organism. Image was generated by Dr. Claudia Florindo, Microscopy Unit, Department of Biomedical and Medical Sciences, University of Algarve, Portugal
	Multi-ciliated cells in Xenopus epidermis. Xenopus laevis stage 28 embryos showing tubulin-GFP expression. Anti-GFP-Alexa 568 antibodies were used for signal amplification. Images were acquired with a Nikon A1R confocal microscope equipped with a 20x NA 0.75 glycerol objective. Stacks were deconvolved with Huygens Essential and visualized with the Huygens MIP Renderer. The red channel shows the GFP fluorescence, the green channel the Alexa 568. Courtesy of Dr. Nicolas Dross, Nikon Imaging Center, University of Heidelberg, Germany
	DVAP aggregates in a Drosophila brain Aggregates in the fly brain formed by a mutant allele of DVAP (Drosophila VAMP associated protein) causing Amyotrophic Lateral Sclerosis (ALS) in humans. Wide-field fluorescence image deconvolved with Huygens Essential. Image was created by Dr. Trudi Gillespie, Membrane Biology Group, University of Edinburgh, United Kingdom
	Living Hela cell, expressing GFP-a-tubulin, mCherry-H2B and SNAP-Actin entering mitosis. Hela cells stably expressing GFP-a-tubulin and mCherry-H2B have been transiently transfected with SNAP-tagged Actin. Cells were stained with appropriate substrate and imaged with Leica SP5 in resonance scanner mode. The acquired z-stack was deconvolved using Huygens Essential software and rendered by the Huygens MIP Renderer. Courtesy of Dr. Grazvydas Lukinavicius, Institute of Chemical Sciences and Engineering, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland
	Nanoparticle uptake by alveolar epithelial cells Huygens Maximum Intensity Projection of a confocal image stack of A549 cells after endocytosis of 11 nanometer silver nanoparticles (green), stained for Lamin B (blue) and Actin (red). The image stack was deconvolved with Huygens Professional, using CMLE and a tri-color measured PSF from TetraSpeck beads. Image was created by Dr. Tobias Mueller, Department of Nano Cell Interactions, INM Leibnitz Institute for New Materials gGmbH, Saarbruecken, Germany

More images can be seen at Image Gallery 1 and Image Gallery 2