What actual resolution improvement can be expected with Huygens?
From the some data the improvement looks like a factor of 10 or 15 times but is this correct & is this typical? I guess the improvement in the Z direction is greater than in the X/Y plane?
As a rule of thumb you get easily 2x in Z and a bit in XY. This is even for poor (noisy) images. If you want more, a measured PSF is necessary. Then you can get up to an apparent resolution in the 100nm range laterally, axially in the 250nm range or better. Of course you need a good signal then. For conventional microscope images with very high SNR the gain is higher, but of course you start at lower resolution. With apparent resolution I mean half-intensity-width (HIW) of a small bead. Some published figures (Bioimaging 4 1996 pp. 187-197):HIW (nm) z x y <br>
raw bead image 790 270 265 <br>
restored 221 116 93 <br>
bead object function 83 83 83 (i.e. the `true' bead) <br>
difference 138 33 10
Conclusion: nearly 4x in Z, more than 2x in XY.
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Keywords: resolution HIW<br>
Categories: Faq Deconvolution, Faq Microscopy, Huygens Faq, Imported Faqs<br>
Platforms: Irix Linux Windows Mac AIX<br>
Related products: Hu Ess Hu Pro<br>
