What emission wavelength to use to represent the detected spectrum?
The detector is seeing a broad spectrum of light, exactly what this is will depend on the filter characteristics prior to the detector.
We once did some simulations where we integrated the PSF(s) over the wavelength band of the dye spectrum and compared that to the PSF of an 'effective' wavelength: a last decimal case. A good estimate of the effective wavelength is the centre of mass of the spectrum, but since the main part of the spectrum is roughly symmetrical and effect of a slight error on the PSF shape is minimal the peak value is also fine.
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Keywords: emission specturm<br>
Categories: Faq Deconvolution, Faq Microscopy, Huygens Faq, Imported Faqs<br>
Platforms: Irix Linux Windows Mac<br>
Related products: Hu Ess Hu Pro<br>
