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Blurring images


We take a simple image to see how it is affected by the application of microscopes Point Spread Functions (PSF). We blur them following the procedure explained in Imaging Simulation. The original image is treated as a XZ (vertical) slice of a Three Dee Image.

Image

Original image


Using the Confocal Microscope PSF the image is blurred, but you can still recognize it:

Image

Confocal blur


Using the Wide Field Microscope PSF you can no longer identify the original object:

Image

Widefield blur



Restoring the images


To obtain a better representation of the original object, we need to do Image Restoration. We apply Huygens Deconvolution in the above blurry images to obtain what follows:

Image

Confocal restored


Image

Widefield restored


Both images are very much improved, but due to the missing cone of frequencies in the widefield case (see Cookie Cutter) horizontal structures (in 3D, horizontal planes, like flat membranes) can not be fully recovered.


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Upcoming Events

1)  Wed 25 of Feb., 2015 09:00 CET
SVI-Huygens at superresolution g-STED symposium Innsbruck
2)  Fri 27 of Mar., 2015 09:00 CET
Huygens imaging course at EMBL Heidelberg
3)  Sun 29 of Mar., 2015 12:21 CEST
SVI at FOM 2015 Goettingen, Germany
4)  Thu 23 of Apr., 2015 09:00 CEST
Huygens imaging course April 2015
5)  Tue 19 of May, 2015 11:59 CEST
Huygens Workshops and Booth at ELMI 2015 Sitges, Barcelona
6)  Wed 10 of June, 2015 15:11 CEST
SVI at Advanced Microscopy and Vital Imaging Maastricht
7)  Tue 01 of Sep., 2015 15:21 CEST
Huygens imaging course September 2015

Contact Information

Scientific Volume Imaging B.V.

Laapersveld 63
1213 VB Hilversum
The Netherlands


Phone: +31 (0)35 64216 26
E-mail: info at svi.nl