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Blurring images


We take a simple image to see how it is affected by the application of microscopes Point Spread Functions (PSF). We blur them following the procedure explained in Imaging Simulation. The original image is treated as a XZ (vertical) slice of a Three Dee Image.

Image

Original image


Using the Confocal Microscope PSF the image is blurred, but you can still recognize it:

Image

Confocal blur


Using the Wide Field Microscope PSF you can no longer identify the original object:

Image

Widefield blur



Restoring the images


To obtain a better representation of the original object, we need to do Image Restoration. We apply Huygens Deconvolution in the above blurry images to obtain what follows:

Image

Confocal restored


Image

Widefield restored


Both images are very much improved, but due to the missing cone of frequencies in the widefield case (see Cookie Cutter) horizontal structures (in 3D, horizontal planes, like flat membranes) can not be fully recovered.


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Contact Information

Scientific Volume Imaging B.V.

Laapersveld 63
1213 VB Hilversum
The Netherlands


Phone: +31 (0)35 64216 26
E-mail: info at svi.nl