Can confocal reflection images be deconvolved? If so, how?
Sometimes one channel of the dataset contains Reflected light signal, i.e., the light which passes straight through various beam splitters and on into the Reflection Detector.
1) Is there any validity in deconvolving this Reflected light component?
2) If so what emission / excitation wavelength's would be applicable?
This is very tricky since reflected light is coherent and full of interference effects. On top of that quite some microscopes have serious trouble with interference between the signal and stray light. In short, deconvolving reflection images is not a good idea. If you still want to proceed, set the excitation and emission values to the wavelength of the reflected light.
Keywords: confocal reflection
Categories: Faq Deconvolution, Faq Microscopy, Huygens Faq, Imported Faqs
Platforms: Irix Linux Mac
Related products: Hu Ess Hu Pro
