Why do I have to center a PSF?
What is 'Center PSF' in the ICTM, Quick-TM and old-Style MLE dialog box mean? I thought a PSF is always in the center.
If the PSF is not centered deconvolution will also shift the
image. Usually this is unwanted, so the PSF generator and current PSF measurement tools always center the PSF. In previous versions of Huygens this was not always the case, so for example the ICTM has an option to center the PSF.
In multi-channel images the different channels are often shifted with respect to each other. By NOT centering the PSF this shift can be automatically undone by the deconvoluttion, and with sub-pixel accuracy.
On the other hand, the shift can be just as well done manually with by the shift tool, also with sub-pixel accuracy. This is perhaps this most practical method.
Since Huygens 3.7 there is a specific option available that measures and corrects for shifts between channels. See Chromatic Shift Corrector.
Keywords: center psf two channel Tikhonov-Miller
Categories: Faq Deconvolution, Huygens Faq, Imported Faqs
Related products: Hu Pro
