Shifted center of mass error message during PSF distillation
During the "Reconstruct psf from bead image" phase in Huygens Professional, or during the PSF distillation in the PSF Distiller, the question "center of mass of the image containing the averaged bead is far (0.482 microns) from the center of the image - proceed anyway" popped up.
This is a check in the Reconstruct PSF tool to prevent the cones (fans) of WF PSFs to be cut off too asymmetrically. Also, this effect can be caused by large spherical aberration making the PSF (and therefore the bead images) highly asymmetrical. This check was built in the software to make people aware of sub-optimal properties of their data which should be corrected during acquisition.
Keywords: center of mass reconstruct psf
Categories: Faq Deconvolution, Huygens Faq, Imported Faqs
Platforms: Irix Linux Windows Mac AIX
Related products: Hu Ess Hu Pro
