How to immobilize a sample with agarose or gelatin
There are many different types of agarose and gelatin, and they may not be equally suited for embedding of light microscopy samples because of autofluorescence, light scattering etc. Are there any special brands of material or protocols/publications for purification/embedding available? Or any other material keeping suspended samples (like beads) in place in three dimensions?
I use gelatin to simply immobilize my sample (suspend it in place in 3-dimensions) which will otherwise tend to "swim away" in an aqueous medium. I do not notice any autofluorescence in the 5% gelatin. <br>
(Answer by Harry Leung, M.Sc.,Technical Officer, Zoology Department, University of Western Ontario, London, Ontario N6A 5B7, E-mail: leung at julian.uwo.ca
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Keywords: agarose gelatin mounting media fluorescent bead<br>
Categories: Faq Microscopy, Huygens Faq, Imported Faqs<br>
Platforms: Irix Linux Windows Mac AIX<br>
Related products: Hu Ess Hu Pro<br>
