Huygens calculates a depth-dependent point spread function (PSF) automatically from the image data set, so no manual interference is necessary. There is also the option to generate (distill) and use an experimental PSF.
Huygens corrects for imaging artifacts such as blurring, noise, bleaching, stability issues, color shifts, and spherical aberration.
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SVI announced STED deconvolution at the FOM 2012
Super-Resolution STED (Stimulated Emission Depletion) microscopic images can now be deconvolved with the new Huygens STED option with truly stunning results. SVI demonstrated STED deconvolution at the FOM meeting in Singapore from April 1-4.
Huygens Confocal and STED deconvolution
Comparision of confocal and STED images, before and after deconvolution with Huygens. Signal shows regulatory proteins Dmc1 and Rad51 for DNA repair during homologous recombination. Dmc1 (green; Mega 520 - 532/685 for ex/em and 770 for depletion), Rad51 (red; Atto 647 640/685 ex/em and 770 for depletion). Courtesy of Dr. Juraj Kabat (Biological Imaging Facility, NIH/NIAID, Bethesda, USA.)
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