Advanced Spherical Aberration Correction Options
Versions of Huygens Core older than 3.3.2 support two advanced correction modes: (1) 'Deconvolution with PSF generated at user-dependent depth' and (2) 'Depth-dependent correction performed on few bricks'. As of Huygens Core 3.3.2 there is a third one: (3) 'Depth-dependent correction performed slice by slice'.
Method (1) uses a static PSF but created at a certain depth (i.e. distance from the coverslip) specified by the user (in the 'Please enter depth for PSF generation (um)' input field). Altough the PSF remains constant during deconvolution, there is a correction performed at creation time as a function of the distance from the coverslip.
Automatic correction with very large refractive index mismatch can result in the Huygens Core breaking down the dataset into a large number of small subvolumes ('bricks'). Artifacts (and a runtime penalty) might appear with increasing number of bricks. Method (2) forces the Huygens Core to use less bricks than it would in automatic mode.
Method (3), in contrast, forces the Huygens Core to deconvolve the dataset one slice at a time.