Using Huygens

You can find here listed different tutorials on using Huygens Software.

Acquisition

Good imaging starts at acquisition, way before image processing.

• Important Factors* for quality restoration
• Acquisition Pitfalls*
• Setting the good sampling: Nyquist Calculator

Restoration

What and what not is Image Restoration. Restoration by Doing Deconvolution.

Essential

• How to use the Huygens Essential wizard: Essential Starting Tutorial*
• Step by step explanation: Essential Deconvolution Roadmap*. Some relevant steps are
  • Estimate Average Background
  • Set The Signal To Noise Ratio

• Batch Processor → Batch Processor Tutorial*
• Deconvolution For Non Optical Images

Deconvolution examples

With detailed explanations on the different parameters involved.

• Example of a Deconvolution Procedure*
• More on Tuning Huygens Deconvolution*

PSF

The Point Spread Function (PSF) contains the most relevant information describing your optical device.

• Recording Beads to obtain an experimental PSF
• Tutorial on Using The Psf Distiller*
• Step by step explanation: Psf Distillation Roadmap*

Professional

All the above tutorials are also relevant for the Huygens Pro. The following ones are more focused in its advanced capabilities:

• Intro Huygens Pro*
• Advanced Huygens Pro*

Visualization and interactive analysis

• Twin Slicer → Twin Slicer Tutorial*
• MIP renderings and animations: Fast Mip
• Realistic 3D rendering: Sfp Renderer → Free SFPTutorial
• Surface Renderer → Surface Renderer Tutorial*
• Movie Maker → Movie Maker Tutorial*
• Colocalization Analyzer → Colocalization Tutorial* and Colocalization Coefficients
• Object Analyzer → Object Analyzer Tutorial*
• Scientific Image Data Base → Sidb Tutorial

Programming

• Introduction to Tcl-Huygens commands
  • Tcl Huygens
  • Intro Hu Pro Before Script*
• How to program Huygens: Scripting Course*
• Some examples: Hu Tcl Useful Procedures*
• Restoration batch jobs: Batch Script*

Presentations

See Huygens Presentations*.

More information

• FAQ
• Huygens Documentation