Restoration Parameters

Also called Deconvolution Parameters in the Huygens Software, the Restoration Parameters are those necessary for Doing Deconvolution, together with the Microscopic Parameters that describe the image.

Restoration Method
Point Spread Function image (if not provided, a theoretical PSF will be used instead based on the Microscopic Parameters).

A typical example for a two-channel image could be:

Restoration Method	Classic Maximum Likelihood Estimation
Point Spread Function	theoretical
Signal To Noise Ratio	{ 14 7 }
Background Mode	Manual
Background Per Channel	{ 900 2400 }
Bleaching Mode	Off
Brick layout	Auto
Quality Change Threshold	0.003
Max Num Of Iterations	80
Iteration Mode	Fast
Padding Mode	auto
Time Out	6000

This article is taken from the SVI-wiki, a support base that also you can edit.

Updated: Fri 15 Dec 2006