Confocal microscope

A Confocal microscope is one type of 3D Fluorescence Microscope in which resolution is increased by rejecting out-of-focus light. This is done by using pinholes with special Pinhole Radius and makes it to be an intrinsic 3D microscope: due to the focusing nature of the confocal microscope, it is specially suitable for obtaining 3D images as a collection of intensities at different points of the space.

A recommendable accessible explanation of how a confocal microscope works: http://www.physics.emory.edu/~weeks/confocal/

In confocal microscopes, the →Nyquist Rate is about one half of that in Wide Field Microscopes.