SVI Image Gallery

Detail of an imaginal disc from a third instar Drosophila melanogaster larva.
Left: a slice of the original data, imaged using an Andor Revolution spinning disc confocal microscope.
Right: the same slice, deconvolved using Huygens Professional.

The fixed sample was stained against alfa-tubulin (green) and gamma-tubulin (red). Recorded by Dr. Paula Sampaio, Advanced Light Microscopy Facility, University of Porto.

Gamma tubulin fiber structure in an imaginal disc.
Left: SFP rendering of the original data, imaged using an Andor Revolution spinning disc confocal microscope.
Right: SFP rendering of the same data, deconvolved using Huygens Professional.

Recorded by Dr. Paula Sampaio, Advanced Light Microscopy Facility, University of Porto.

Macrophage fluorescently stained for tubulin (yellow), actin (red) and the nucleus (DAPI, blue).
Left: original image, recorded with a wide field microscope.
Right: the same dataset, deconvolved using Huygens Professional.
The datasets are visualized with top-view maximum intensity projections.
Data courtesy of Dr. James Evans, Whitehead Institute, MIT Boston MA, USA. (See the Evans Macrophage for more image details).

Cell nuclei labelled with Draq5 (XY slice).
Left: a slice of the original data, imaged using an inverted Leica TCS SP2 AOBS confocal microscope.
Right: the same slice, deconvolved using Huygens Professional.

This image was used to test an automatic nuclei counting algorithm. Huygens deconvolution enhances the performance of this method. Recorded by Dr. Nicolas Fête, Laboratory of Stem Cells Dynamics, Swiss Federal Institute of Technology of Lausanne.

Cell nuclei labelled with Draq5 (axial XZ slice).
Left: a slice of the original data, imaged using an inverted Leica TCS SP2 AOBS confocal microscope.
Right: the same slice, deconvolved using Huygens Professional.

This image was used to test an automatic nuclei counting algorithm. Huygens deconvolution enhances the performance of this method. Recorded by Dr. Nicolas Fête, Laboratory of Stem Cells Dynamics, Swiss Federal Institute of Technology of Lausanne.

3D SFP rendering of a cell cluster.
The XY and XZ slices in the previous images are part of this dataset.

In the SFP volume rendering algorithm the data is taken as a distribution of fluorescent dye. By modeling a physical light/matter interaction process an image is computed showing the data as it would have appeared in reality when viewed under these conditions. The original data is recorded by Dr. Nicolas Fête, Laboratory of Stem Cells Dynamics, Swiss Federal Institute of Technology of Lausanne.

Axial image of an isolated Rat Hepatocyte couplet.
Left: a XZ slice of the original confocal 3D image.
Right: the same XZ slice after restoring the whole stack with the Huygens Software.

The data shows two adhering liver cells stained with phalloidin for actin (red), tubulin (blue) and dextran as a marker for endocytosis (green). Data recorded with a confocal microscope by Dr. Permsin Marbet at the Department of Anatomy, University of Basel, Switzerland, in the lab of Prof. Lukas Landmann. For more views of this dataset, see the SVI wiki.

Axial 4Pi two-photon image of F-actin filaments of a mouse skin fibroblast cell.
Left: a 'conventional' confocal image.
Right: the restored 4Pi two-photon image.

Both 3D-images were recorded at the same site of the specimen to allow a direct comparison of both methods. The F-actin fibers are directed along the Y-axis, i.e., perpendicular to the axial image. The 4Pi-confocal restored image reveals more details of the object than the confocal counterpart as a result of the improved 3D-resolution. The actin fiber in the center is substantially better resolved. The axial FWHM resolution in the restored 4Pi image was shown to be 70nm. Data courtesy of Prof. Stefan W. Hell, Nano Biophotonics group, Max Planck Institute.