I have heard that imaging diatoms is a difficult task, why?
I intend to obtain some diatoms which have detail in the sub-micron range and which have 3-d structures. It should be possible to image them by immersing them in a fluorescent medium and model their appearances using different algorithms to find which one gives the best approximation of the real appearance of the diatom (as determined by scanning EM).
Imaging diatoms is indeed a difficult task. This is so because the skeleton of diatoms has a high refractive index and can seriously spoil light propagation, resulting in a PSF which varies strongly from place to place.
Keywords: diatoms
Categories: Faq Deconvolution, Faq Microscopy, Huygens Faq, Imported Faqs
Platforms: Irix Linux Windows Mac
Related products: Hu Ess Hu Pro
