Using Huygens

This page lists useful Huygens Software tutorials.

Acquisition

Good imaging starts at acquisition, way before image processing.

• Important Factors for quality restoration
• Acquisition Pitfalls
• Setting the good sampling: Nyquist Calculator

Restoration

What and what not is Image Restoration . Restoration by Doing Deconvolution .

Essential

• How to use the Huygens Essential wizard: Essential Starting Tutorial
• Step by step explanation: Essential Deconvolution Roadmap . Some relevant steps are
  • Estimate Average Background
  • Set The Signal To Noise Ratio

• Batch Processor → Batch Processor Tutorial
• Deconvolution For Non Optical Images

Deconvolution examples

With detailed explanations on the different parameters involved.

• Example of a Deconvolution Procedure
• More on Tuning Huygens Deconvolution

PSF

The Point Spread Function (PSF) contains the most relevant information describing your optical device.

• Recording Beads to obtain an experimental PSF
• Tutorial on Using The Psf Distiller
• Step by step explanation: Psf Distillation Roadmap

Professional

All the above tutorials are also relevant for the Huygens Pro. The following ones are more focused in its advanced capabilities:

• Intro Huygens Pro
• Advanced Huygens Pro

Visualization and interactive analysis

• Twin Slicer → Twin Slicer Tutorial
• MIP renderings and animations: Fast Mip
• Realistic 3D rendering: Sfp Renderer → Free SFPTutorial
• Surface Renderer → Surface Renderer Tutorial
• Movie Maker → Movie Maker Tutorial
• Colocalization Analyzer → Colocalization Tutorial and Colocalization Coefficients
• Object Analyzer → Object Analyzer Tutorial
• Scientific Image Data Base → Sidb Tutorial

Programming

• Introduction to Tcl-Huygens? commands
  • Tcl Huygens
  • Intro Hu Pro Before Script
• How to program Huygens: Scripting Course
• Some examples: Hu Tcl Useful Procedures
• Restoration batch jobs: Batch Script

Presentations

See Huygens Presentations .

More information

• FAQ
• Huygens Documentation

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