Does Huygens deconvolve Transmission images?
Yes, Huygens will certainly improve the image.
The first test should be done using a Wide Field Microscope Theoretical Psf with the
actual parameters used for the acquisition, maybe with a lower Numerical Aperture to
enlarge the PSF a little bit.
Still, remember that deconvolution assumes that the Image Formation is
linear, and transmission is not, due to possible interference effects. These effects are lesser for thicker samples, but they can create restoration artifacts if they are noticeable. These can be balanced by properly tuning the Signal To Noise Ratio and the Max Num Of Iterations.
The ideal particle to distill an Experimental Psf with the Psf Distiller is probably a sub-resolution gold particle.
Remember that all the images should be inverted (negative) in Huygens before distilling a PSF and deconvolution: high intensities (gray values closer to white) should describe high object density, but raw transmission images provide the opposite. In Huygens Essential, this can be done with Tools > Invert image.
The Ideal Sampling constraints are about half as stringent as for the confocal imaging mode. For an objective with high N.A. (1.4) the voxel sizes should be in the range of 100-150 nm laterally and 350-500 nm axially.
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Keywords: transmission image<br>
Categories: Faq Deconvolution, Huygens Faq, Imported Faqs<br>
Platforms: Irix Linux Windows Mac AIX<br>
Related products: Hu Ess Hu Pro<br>
