Can I deconvolve images acquired with a TIRF system?
TIRF (Total Internal Reflection Fluorescence) deals mostly with 2D images. Huygens can handle 2D images by internally treating them as part of a 3D stack from which most planes happen to be missing.
Strictly speaking Huygens does not generate TIRF Theoretical PSFs, but customers report good results with high NA confocal PSFs. Proceed by setting the Microscope type of your image to confocal and NA ~ 1.4.
An experimental PSF, if existing, can naturally be used.
See wiki article → Total Internal Reflection.
Keywords: TIRF Total Internal Reflection Fluorescence PSF 2D
Categories: Faq Deconvolution, Huygens Faq, Imported Faqs
Platforms: Irix Linux Windows Mac AIX
Related products: Hu Ess Hu Pro Hu Script
