Can I deconvolve images acquired with a TIRF system?
Compatibility with TIRF (Total Internal Reflection Fluorescence) systems
TIRF (Total Internal Reflection Fluorescence) is mostly 2D imaging. Huygens can handle 2D images by internally treating them as part of a 3D stack from which most planes happen to be missing.
Strictly speaking Huygens does not generate TIRF Theoretical Psfs, but customers report good results with high NA confocal PSFs. Try by setting the Microscopic Parameters of your image to Confocal Microscope and NA ~ 1.4.
If you manage to measure an experimental PSF, you can always use it directly.
See wiki article → Total Internal Reflection.
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Keywords: TIRF Total Internal Reflection Fluorescence PSF 2D<br>
Categories: Faq Deconvolution, Huygens Faq, Imported Faqs<br>
Platforms: Irix Linux Windows Mac AIX<br>
Related products: Hu Ess Hu Pro Hu Script<br>
