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Is there any way to read a 4D tiff image series into Huygens Essential or Huygens Pro?


If the Tiff series is numbered with Leica style extentions it is read as a 3D multichannel timeseries. So instead of just a sequential numbering they should be numbered like:
psa-880_Series08_t00_z000.tif
psa-880_Series08_t00_z001.tif
...
psa-880_Series08_t01_z000.tif
psa-880_Series08_t01_z001.tif
...
psa-880_Series08_t09_z027.tif
psa-880_Series08_t09_z028.tif

Huygens Pro has a lot of conversion utilities and tools to shuffle frames and channels around. See Operation window -> Convert

Lastly it is possible to edit the ICS file like:
layout  parameters      4
layout  order   bits    x       y       z
layout  sizes   8       256     256     64
to:
layout  parameters      5
layout  order   bits    x       y       z       t
layout  sizes   8       256     256     16      4

The only tricky thing is that the field separators must be tabs, not spaces.



Keywords: tiff 4D files
Categories: Faq Files, Huygens Faq, Imported Faqs
Platforms: Linux Windows Mac
Related products: Hu Ess Hu Pro Hu Script