Can a small sphere-like object look again like a sphere in X Y Z after deconvolution?
The restoration improves the resolution in all directions, but more so in the z-direction. In typical confocal images it is easy to increase the z-resolution by a factor 2. With a measured PSF a factor 4 is attainable.
However, the z-resolution in the measured image is often 4x worse than the lateral resolution. So at best you can compensate for that, but due to the lateral resolution gain the result will still be non-spherical. The gain in lateral resolution can be spoiled by applying a Gaussian filter (Operation window -> Restoration -> Gaussian filter/Quick Gaussian filter) to decrease lateral resolution again in a controlled manner. Because, in our experience, practically no one wishes to reduce resolution the restoration tools do not do that automatically.
Still, if the sphere is physically small enough (of the order of a voxel) the restoration can reduce it to close to a single voxel. Provided that the z-sampling is small enough. This situation occurs easily in deconvolution of widefield images with 100nm x 100nm x 100nm voxel size.
Keywords: spheres isotropic resolution
Categories: Faq Deconvolution, Faq Microscopy, Huygens Faq, Imported Faqs
Platforms: Irix Linux Windows Mac AIX
Related products: Hu Ess Hu Pro
