What is more "dangerous" undersampling or oversampling?
What is more "dangerous" i.e. leading to false deconvolution results and / or hugely images : undersampling or oversampling? To which direction (lateral or vertical) and to which extent?
Oversampling is completely harmless. The more samples the better, though it could be argued that oversampling is not *necessary*. Of course there are practical reasons to limit sampling: object size, memory requirements, bleaching and so on.
If you have loaded an image in Huygens Pro, select it and select Edit-> Nyquist you get a report of the sampling situation taking into account all known parameters. Rule of thumb is for WF: 100nm lateral or better; around 200nm axially. 250nm would be fine too, but we distrust some z-motors with round numbers.
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Keywords: sampling oversampling<br>
Categories: Faq Deconvolution, Faq Microscopy, Huygens Faq, Imported Faqs<br>
Platforms: Irix Linux Windows Mac<br>
Related products: Hu Ess Hu Pro<br>
