Is there a mismatch between the refractive index of the bead and the surrounding medium?
Am I right in assuming, that because microspheres are spherical, a mismatch between the refractive index of the bead and the surrounding medium is of no significance?
What happens when you use a small bead with slightly higher r.i. than the surrounding medium to measure the PSF that it will be not quite homogeneously excited. I think this effect on beads with a size approximately equal to the size of the diffraction spot in XY and so much smaller than the diffraction spot in Z can be neglected. The emitted light from within the sphere might seem to come from outside the sphere because of the sphere acting as a lens, but this is at most something in the order of diameter*( RI_bead/RI_lens -1) outside the sphere, so something like 10nm, also neglectable.
Keywords: refractive index mismatch measuring beads
Categories: Faq Deconvolution, Huygens Faq, Imported Faqs
Platforms: Irix Linux
Related products: Hu Pro
