Out of memory problem when generating a widefield PSF?
1. Close images you are not immediately working on and turn off the Undo system.
2. Use the crop tool to crop the data as much as possible, especially in the Z direction.
3. Use either QMLE or CMLE to deconvolve the image. If possible both will split the data into bricks and generate PSFs matching the bricks. In this way the computation of a single huge PSF is avoided. Currently, widefield images can only be processed brick wise if the data is sufficiently shallow compared to the NA. This is the case when the base of the aperture cone as truncated by the upper and lower planes is far smaller than the lateral extent of the data. This is often true, but overestimated NAs can spoil it. It helps to cut off as many z-planes as possible since this not only reduces the data size, but also allows more efficient brick cutting.
4. Lastly, make sure your system has sufficient swap space.
Keywords: PSF memory widefield
Categories: Faq Deconvolution, Huygens Faq, Imported Faqs
Platforms: Irix Linux Windows Mac AIX
Related products: Hu Ess Hu Pro Hu Script
