Should I use 175 nm or 260nm diameter beads to measure the PSF?
With large beads the software has a much harder job extracting a PSF than from images of small beads. However, small beads are noisier and will need some averaging to reach a sufficient Signal to Noise Ratio (SNR). Very small low signal beads can't be averaged because their position cannot be measured with sufficient accuracy. <br>
As a rule 175 nm diameter beads yield sufficient signal while being small enough. We have seen good results with 110nm beads, but we do not think these can be imaged with sufficient SNR by all microscopes. <br>
For high NA microscopes (NA > 1.2) we do not recommend beads as large as 260nm.
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Keywords: PSF beads size<br>
Categories: Faq Deconvolution, Faq Microscopy, Huygens Faq, Imported Faqs<br>
Platforms: Irix Linux Windows Mac AIX<br>
Related products: Hu Ess Hu Pro<br>
