Is there a suitable source of multi-wavelength micro-beads for PSF measurements?
Dr. Markus Duerrenberger designed a confocal calibration kit, now available from Polysciences. This kit contains three different sizes of latex beads 0.2mu, 0.5mu and 1mu (all +/- 0.001mu), fluorescent in the whole range of wavelength from 280nm up to 647nm. These beads work extremely well for the Huygens Pro, but also all kinds of alignments and adjustments of the microscope can be done in a highly precise way.
Also Invitrogen and Duke Scientific have good beads available. See also BeadsMicrospheresImpFaq
Keywords: multi-wavelength micro-beads PSF measurements
Categories: Faq Microscopy, Huygens Faq, Imported Faqs
Platforms: Irix Linux
Related products: Hu Pro
