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SVI Movie Gallery 3






BEM-1-GFP at septum of Neurospora crassa hyphae.

BEM-1-GFP localizes at the septa of Neurospora crassa hyphae. The function of BEM-1 in N. crassa is still unknown. Stack of widefield images taken with a Deltavision (AP) Spectris DV4; Objective: Olympus 100X/1.40, Plan Apo, IX70; cooled Camera: CH350 / KAF1400. Images were deconvolved with Huygens. Courtesy of Dr. Timo Schuerg at the Department of Genetics, TU Braunschweig, Germany







Mitochondrial dynamics in human fibroblast.

3D Times series of spinning disk images, deconvolved and rendered in Huygens. Z-stack taken every 20 seconds over half an hour of a live human fibroblast expressing a mitochondrially targeted red fluorescent protein. The image shows part of the mitochondrial network in an old fibroblast's cytoplasm and displays how much movement, fusion and fission happens over time. Courtesy of Dr. Glyn Nelson, University of Newcastle upon Tyne, United Kingdom.







Recruitment DsRed2-PKD1 (red channel) to the immune synapse is more obvious after deconvolution.

The two movies show a raw (1st movie) and Huygens deconvolved (2nd movie) image series of DsRed2-PKD1 in a human Jukat T lymphocyte (red channel). The blue channel in both movies was not deconvolved and shows CMAC as a cell tracker. Images were recorded with a NIKON TiE (Plan APO VC Objective 60x, 1.4 NA).
Recruitment of DsRed2-PKD1 from the cytosol to the immune synapse occurs after the Jurkat cell recognizes and binds to the Raji B lymphocyte. This recruitment is much more obvious after deconvolution. Courtesy of Dr. Manuel Izquierdo, CSIC-Universidad Autónoma de Madrid, Spain.







Tetrahymena with cilia and Mob1 staining.

Tetrahymena(external link) was stained for centrin (red) and Mob1-GFP (green). Stacks were acquired with a Delta Vision microscope and images were subsequently deconvolved with Huygens. The movie was created with the Huygens Surface Renderer and Movie Maker. Centrin staining is present in the cilia of Tetrahymena, and Mob1 staining is found in the apical region of this organism. Courtesy of Dr. Claudia Florindo, Microscopy Unit, Department of Biomedical and Medical Sciences, University of Algarve, Portugal