SVI Movie Gallery 2
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Metaphase cell with kinetochores (red) attached to microtubules (green).
Cells were stained with a-tubulin (green), cenp-A (red) and DAPI (blue). Stacks where acquired with a Delta Vision (widefield) microscope, deconvolved with Huygens Professional. The movie was created with the Surface Renderer in Huygens. It can be observed that in a metaphase cell
kinetochores (red) attach to microtubules. Courtesy of Dr. Claudia Florindo, Microscopy Unit, Department of Biomedical and Medical Sciences, University of Algarve, PortugalFlash player not available.
DiI-labeled spiny dendrite.
Neurons were labeled with the cyanine membrane dye DiI. Image stacks were recorded with a 1.4 NA oil immersion objective, and deconvolved using Huygens. The deconvolved 3D time series was rendered with the SFP renderer and saved as a movie file with Huygens. Courtesy of Barna Dudok, Molecular Neurobiology, Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, Hungary.Flash player not available.
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Living HeLa cell expressing GFP-a-tubulin, mCherry-H2B and SNAP-Actin entering mitosis
HeLa cells stably expressing GFP-a-tubulin and mCherry-H2B have been transiently transfected with SNAP-tagged Actin. Cells were stained with appropriate substrate and imaged with a Leica SP5 in resonance scanned mode. Acquired z-stack were deconvolved using Huygens Essential software and represented as a movie generated by the SFP Renderer. Courtesy of Dr. Grazvydas Lukinavicius, Institute of Chemical Sciences and Engineering, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, SwitzerlandFlash player not available.
Human chondrocyte stained for TSG-6 and Hyaluronan.
A chondrocyte within human cartilage was stained with an antibody specific for TSG-6 (Tumour Necrosis Stimulated Gene-6; secondary antibody is conjugated to Alexafluor 555) and probed with biotinylated Hyaluronan-Binding protein (b-HABP) to identify Hyaluronan (HA). 3D time images were deconvolved and subjected to the Surface Renderer in Huygens. Courtesy of Dr. Sheona Drummond, Wellcome Trust Centre for Cell Matrix Research, Faculty of Life Sciences, University of Manchester, United Kingdom.