MLE versus ICTM - Will one method be faster or more rigorous or give a better quality result?
For fluorescence and from a theoretical viewpoint MLE restoration is the only proper choice. However, for high SNR's as in widefield images this becomes rather academic. Since the ICTM method in Huygens Pro is computationally more efficient than the MLE implementation we suggest using ICTM for WF images. As to `quality' that is or a subjective criterion based on visual inspection (ICTM might win in low noise cases) or it is based on a mathematically sound criterion (MLE proven to be best choice). For noisy images (most confocal ones) the MLE is not only scientifically more correct, it also produces visually more pleasing images: far less background noise artifacts than ICTM.
Keywords: MLE ICTM SNR
Categories: Faq Deconvolution, Huygens Faq, Imported Faqs
Platforms: Irix Linux
Related products: Hu Pro
