How do I embed beads in water for PSF measurements?
Basically it is very simple: beads were brought on a coverslip and left to dry. Then for water type medium simply distilled water was added. For high refractive index Aquatex (Merck) was added, covered with a second coverslip and left to dry for 3-4 weeks. For this last sample 115 nm beads from Molecular probes were used with very good results. For a recipe using glycerol see section "Measuring a PSF" in the recipe booklet that you received in the case you have purchased the software.
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Keywords: measuring psf glycerol coverslip<br>
Categories: Faq Deconvolution, Faq Microscopy, Huygens Faq, Imported Faqs<br>
Platforms: Irix Linux Windows Mac AIX<br>
Related products: Hu Ess Hu Pro<br>
