How critical is the difference between the nominal size of the microsphere and its actual size?
Manufacturers supply the average size along with a standard deviation. Should we look for very tightly specified beads?
The spheres we developed and tested this technique with, had a coefficient of variation of 2% at 230nm diameter. This variation was smaller that the precision at which we could locate the center of the beads, at best 1/4 pixel. So if the c.v. is a good deal better than say 1/4 of a pixel it is good enough.
Keywords: measured beads
Categories: Faq Deconvolution, Huygens Faq, Imported Faqs
Platforms: Irix Linux
Related products: Hu Pro
