SVI Image Gallery 2

Image	Description
	Surface rendered 4Pi image of synaptonemal complex 3. Synaptonemal complex 3 in a diplotene mouse spermatocyte. 4Pi microscope image deconvolved and rendered with the Surface Renderer in Huygens. Image created by Dr. Gert van Cappellen. Dept. of Reproduction & Development, Erasmus MC, The Netherlands
	Phosphorylation at Serine 10 of histone H3 is tightly correlated with chromosome condensation during mitosis. Endogenous proteins in Hela cells were stained with anti-beta-tubulin Cy3 coupled (red), anti-pH3 ser10 (green). Nuclei were stained using Hoechst 33342 (blue). Cells were imaged with a Leica TCS SP5 confocal scanner and a 63x/1.32NA objective. Deconvolution was achieved with Huygens. Image created by Dr. Cedric Boularan, Laboratory of Immunoregulation, NIH, NIAID, Bethesda, USA
	Cell images from multi-epitope-ligand-cartography analysis. Immortalized cerebellar granular cells (Cb) are sequentially labeled with 35 FITC-conjugated tags against subcellular components and visualized with Zeiss 200M wide-field microscope using a Toponome Imaging System. Background corrected image data from 7 tags is shown before and after deconvolution with Huygens Professional (top row). Cropped region subjected to the Huygens Surface Renderer shows individual signals (bottom). Image created by Dr. Mika Ruonala, Center for Membrane Proteomics, Goethe University of Frankfurt am Main, Germany
	Multi-ciliated cells in Xenopus epidermis. Xenopus tropicalis stage 35 tailbud embryos were fixed and stained for acetylated tubulin which highlights cilia in the epidermis. The nuclear stain is Draq5. Images were acquired with a Nikon A1R confocal laser scanning microscope equipped with a 60x NA 1.4 objective. Stacks were deconvolved with Huygens Essential and subsequently rendered with the Simulated Fluorescence Process Renderer. Image created by Drs. Nicolas Dross and Ulrike Engel, Nikon Imaging Center, University of Heidelberg, Germany
	Multiciliated cells in Xenopus epidermis. Xenopus tropicalis stage 35 tailbud embryos were fixed and stained for acetylated tubulin which highlights cilia in the epidermis. The nuclear stain is Draq5. Images were acquired with a Nikon A1R confocal laser scanning microscope equipped with a 60x NA 1.4 objective. Stacks were deconvolved with Huygens Essential and subsequently rendered with the Huygens Surface Renderer. Image created by Dr. Nicolas Dross and Ulrike Engel, Nikon Imaging Center, University of Heidelberg, Germany
	Neuronal synapse. Neuronal terminal (green) on GABA-labeled neuron cell membrane (red). Image width approx 5 microns. Image has been deconvolved with Huygens. Image created by Dr. Ray Gilbert, Center for Brain Research, Faculty of Medical and Health Sciences, University of Auckland, New Zealand
	Swine ulnar growth plate. Image taken with a Zeiss LSM510 with a Plan-Neofluar 10x/0.3NA objective represents growth plate proliferative zone labeled with Safranine O. Image was deconvolved with Huygens. Image created by Samira Amini MSc., Department of Mechanical Engineering, Ecole Polytechnique of Montreal, Québec, Canada
	Deconvolved and SFP rendered image of a dendritic tree of a cultured hippocampal neuron. Primary hippocampal neuronal culture from P1 C56Bl/6H mice, grown on glass bottom dishes, imaged live in aqueous medium, expressing CMV-driven mCherry (red) and mouse diacylglycerol lipase (with pAcGFP, green) Images were recorded with 1,4 NA 60x oil immersion objective on a Nikon Ti-E inverted microscope using a spectral detector. Image recorded by Barna Dudok MSc., Hungarian Academy of Sciences, Hungary
	Visualization of engineered nanoparticles localized inside a macrophage. Mono-cultures of human blood monocyte-derived macrophages were incubated for 24 h with polymer coated fluorescently labeled iron-platinum nanoparticles (show in pink color). Cells were stained with a cell-labeling dye (light blue) and MitoTracker® (dark blue) which specifically label the mitochondria. Live cells were imaged by confocal laser scanning microscopy, deconvolved with Huygens, and visualized with the Huygens Simulated Fluorescence Process Renderer. Image created by Dr. Barbara Rothen, Institute of Anatomy, University of Bern, Switzerland
	BEM-1-GFP at septal pore of N. crassa hypha. The function of BEM-1 in Neurospora crassa is still unkown. The bottom right picture shows a 3D-reconstruction of the septal pore which is rotated about 90°. Bright/Wide-field image stacks were recorded with Zeiss Examiner.D1 with 100X/1.30NA oil objective, and deconvolved with Huygens. Images were recorded by Dr. Timo Schuerg at the Department of Genetics, TU Braunschweig, Germany
	Trypanosoma cruzi-infected Vero cells. Vero cells infected with T. cruzi were labeled with two monoclonal antibodies: 3B2 (shown in green) reacts with the flagellated trypomastigote forms, and 4B5 (in red) recognizes an intracellular structure in all parasite forms. DAPI (in blue) was used to stain all DNA rich organelles - kinetoplasts and nuclei. Serial Z sections acquired on a BioRad confocal microscope were deconvolved and SFP-rendered using Huygens. Image recorded by Dr. Renato Mortara, Department of Parasitology, Escola Paulista de Medicina, Sao Paolo, Brazil
	Multicolored bead image. Compilation of three 500nm beads (in 2 colors) and one 200nm bead in four colors. Image was rendered with the Huygens Simulated Fluorescence Process Renderer. Note that the chromatic shift is clearly visible. Image created by Dr. Mariette Kemner- van de Corput, Department of Cell Biology and Genetics, Erasmus Medical Center, Rotterdam, The Netherlands
	Mitochondrial dysfunction in senescent fibroblasts. 20x air (0.7 NA) widefield image captured on a Leica upright of living human fibroblast cells stained to show the mitochondrial network (green) and it's activity (red) and cell nuclei (blue, using mitotracker green, TMRM and Hoechst respectively). Z stack fluorescent channels were deconvolved in Huygens Essential and rendered using SFP. Individual cells can be seen to have varying levels of energy production (red intensity), as well as intracellular variation between individual mitochondrial networks. Image created by Dr. Glyn Nelson, University of Newcastle upon Tyne, United Kingdom
	Blood platelet. A three-dimensional representation of a blood platelet (green) filled with red granules of von Willebrand factor. Image from the labs of Drs. Jerry Ware and Brian Storrie in the Department of Physiology & Biophysics, University of Arkansas for Medical Sciences, Little Rock, USA

More images can be seen at Image Gallery 1 and Image Gallery 3