The PSF looks "banana-shaped" in the x-z or y-z planes. What could be the reason?
I measured PSF with beads using a 100x, 1.40-oil objective. The PSF looks clearly banana-shaped in the x-z or y-z planes. Do you believe that this is only because of the objective or if it can be due to any misalignment in the scanner? I use the appropriate immersion oil and cover glass.
We have seen this many times. Please see Banana Psf.
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Keywords: banana-shaped PSF pinhole scan lens<br>
Categories: Faq Microscopy, Huygens Faq, Imported Faqs<br>
Platforms: Irix Linux<br>
Related products: Hu Pro<br>
