Averaging beads - image size criteria is too small.
What happens is when I go to average beads, a message saying the axial and/or lateral image size criteria is too small. It then removes the axial size criterium. Is this OK??
The message about the axial criterium may mean that the beads are too close to the top and bottom edges. In that case a bead below or above might disrupt the PSF. Since usually the beads are adherent to the glass there is little danger of this so you can ignore it.
But it may also mean that the image is indeed having not enough information about the PSF along the optical axis Z. The recorded volume around beads, specially in widefield microscopes, must be large enough to containg information about the light cone. If this is the case, better image the beads again, maybe changing the sampling density and recording more planes. See http://support.svi.nl/wiki/RecordingBeads
.Keywords: averaging beads image size
Categories: Faq Deconvolution, Faq Microscopy, Huygens Faq, Imported Faqs
Platforms: Irix Linux
Related products: Hu Pro